Research Article
Use of a Multiplex PCR for the Detection of Toxin-Encoding Genes netB and tpeL in Strains of Clostridium perfringens
Table 3
BLAST output for sequenced PCR products*.
| | Target gene | Sequence source† | Description of top result | GenBank accession | Max identity (%) | -value |
| | netB | C103-99 | Clostridium perfringens strain CP4 plasmid pCP4netB pathogenicity locus 1 genomic sequence | JF837812.1 | 100 | | | netB | JGS-1870 | Clostridium perfringens strain CP4 plasmid pCP4netB pathogenicity locus 1 genomic sequence | JF837812.1 | 100 | | | netB | JGS-4140 | Clostridium perfringens strain CP4 plasmid pCP4netB pathogenicity locus 1 genomic sequence | JF837812.1 | 100 | | | tpeL | C103-99 | Clostridium perfringens A strain CP4 TpeL (tpeL) gene, complete cds | EU848493.1 | 99 | 0 | | tpeL | JGS-1870 | Clostridium perfringens A strain CP4 TpeL (tpeL) gene, complete cds | EU848493.1 | 99 | 0 | | tpeL | JGS-4140 | Clostridium perfringens A strain CP4 TpeL (tpeL) gene, complete cds | EU848493.1 | 99 | 0 |
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Each row corresponds to a single sequenced band.
†lists the strain that the sequenced band was amplified from.
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