Effect of G. brevis extract (GBE) on clonidine-induced catalepsy. 8–10-week-old ICR mice (20–30 g) received clonidine 1 mg · kg⁻¹, s.c. and their forepaws placed on a horizontal bar (1 cm in diameter, 10 cm above the table). The duration of catalepsy was measured at 30 min intervals up to 180 min after administration of clonidine. (a) In the preventive (prophylactic) protocol, vehicle (5 mL kg⁻¹), GBE (30, 100, and 300 mg kg⁻¹), and chlorpheniramine (10 mg kg⁻¹) were given orally for 2 consecutive days ending 30 min before clonidine injection. (b) In the curative (therapeutic) protocol drug treatment commenced 1 h after induction of catalepsy. Values are mean ± s.e.m. (). Data was subjected to two-way (treatment × time) repetitive measures analysis of variance (ANOVA) with Bonferroni’s post hoc test. Significance between groups of vehicle and drug/extract-treated mice ***, **, *, ns is nonsignificant.