NK-1R immunoreaction patterns in nerve fascicles. Sections of soleus muscle specimens from the control group (a), the 1-week group, exercised side (b) and the 6-week group, nonexercised side (c) and of gastrocnemius muscle specimens from the 6-week group, exercised side (d) and nonexercised side (e, f). The nerve fascicle shown in (b) was located in a region showing normal muscle morphology whilst those shown in (c)–(e) were localized to myositis areas. Note that there are no NK-1R immunoreactions in the nerve fascicle in (a). This fascicle is seemingly entirely composed of myelinated nerve fibers. There are also no immunoreactions in the nerve fascicles in (b) (above and below). Just outside these, there are fine varicose immunoreactive nerve fibers (arrows). On the other hand, there are marked NK-1R immunoreactions in the nerve fascicles shown in (c)–(e) (arrows). These show a punctuate appearance, with the reactions to some extent, however, being spread out in broad areas. In (f), a section parallel to the section shown in (e) is seen, and for which the NK-1R antibody had been preabsorbed with NK-1R peptide. There are no NK-1R immunoreactions in the nerve fascicle in (f). Asterisks mark corresponding regions in (e) and (f). Bars = 25 μm.