Anti-peptide antibody levels elicited by NS4b mimotope as MAP. Serum samples were obtained on day 0 (preimmune) and after four injections (day 56) from mice immunized with 50 μg of antigen (NS4b MAP). The reactivity of each serum was individually tested, and the average of the results is shown. ELISA plates were coated with the homologous peptide, and sera were diluted 1 : 50. Results are expressed as OD ratio and are presented for OD492 nm (day 56)/OD492 nm (preimmune sera). The cutoff value is an OD ratio ≥2.0. Antibody levels against NS4b as MAP were significantly higher () than the PBS control.