Research Article

Overexpression of Regulatory T Cells Type 1 (Tr1) Specific Markers in a Patient with HCV-Induced Hepatocellular Carcinoma

Table 1

Primer sequences used in real-time quantitative RT-PCR assay.

Gene Primers sequencesAnnealing temperature (°C)

hCD4:5′-GGGAAATCAGGGCTCCTTCTTA
5′-TGGTCCCAAAGGCTTCTTCTT
59
hCD25:5′-GGGACTGCTCACGTTCATCA
5′-TTCAACATGGTTCCTTCCTTGTAG
59
hCD49b:5′-CAACGGGTGTGTGTTCTGACA
5′-TCATCACACACAACCACAACATCT
59
hCD18:5′-ATGCTTGATGACCTCAGGAATGT
5′-ACGGTCTTGTCCACGAAGGA
59
hT1-ST2:5′-GTGTTTGCCTCAGGCCAACT
5′-TGACATTCGCATATCCAGTCCTA
59
hCCR5:5′-GTCAAGTCCAATCTATGACATCAATTATT
5′-CGGGCTGCGATTTGCTT
57
hCD8 :5′-CCCTGAGCAACTCCATCATGTAC
5′-GGCGTCGTGGTGGGC
60
hCD8 :5′-TGGCCGCGCAGCTG
5′-CTTGTTGGTTTGCACCTTTATGTATG
55
hCD19:5′-CTCACCCCCATGGAAGTCAG
5′-CTTGAGGCACTGCAGCACAG
60
hCD11c: 5′-AATTCAGGCGCACGTCAAA
5′-ATCCCTACGGGCCCCATAT
56
hICAMI:5′-CCCTGATGGGCAGTCAACA
5′-GCAGCGTAGGGTAAGGTTCTTG
60
h -actin:5′-CACGGCATCGTCACCAACT
5′-AGCCACACGCAGCTCATTG
58
hIL-4:5′-CACAAGCAGCTGATCCGATTC
5′-TTCCAAGAAGTTTTCCAACGTACTC
59
hIL-2:5′-ACCAGGATGCTCACATTTAAGTTTTAC
5′-TCCAGAGGTTTGAGTTCTTCTTCTAGA
61
hIL-10:5′-GAGAACCAAGACCCAGACATCAA
5′-CCACGGCCTTGCTCTTGTT
59
hIL-10R :5′-CCGAGAGTATGAGATTGCCATTC
5′-CAGATGGTTTCACCTGGACACA
60
hIL-10R :5′-TGGGAGTCACCTGCTTTTGC
5′-TCCGTCAAGGTAGTATTCATGCA
59
hIFN :5′-ATGTAGCGGATAATGGAACTC
5′-GACATTCAAGTCAGTTACC
53
hTGF- 1:5′-CGAGCCTGAGGCCGACTAC
5′-CGGAGCTCTGATGTGTTGAAGA
62
hTGF- R1:5′-TGACAACGTCAGGTTCTGGCT
5′-AATCGACCTTTGCCAATGCT
57
hTGF- R2:5′-GCTGCTTCTCCAAAGTCATT
5′-AACAAGTCAGGATTGCTGGTGTT
58
hP-Selectin:5′-CTGGAACCCCTGAGTCTACCAC
5′-GTCTGTATCTCCATAGCTGCTGAATC
63
h5′G3PDH:5′-CCATCAATGACCCCTTCATTG
5′-CTTGACGGTGCCATGGAATT
58