Extrahepatic 25-Hydroxylation of Vitamin D3 in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development
Figure 5
ALP activity of OPC1 and MC3T3-E1 cells maintained in six types of tissue culture medium aggregated over three two-week periods (). After two weeks, the OPC1 ALP activity of 1,25OH2D3 and osteogenic factors (BM+) treated cells were statistically greater when compared to the negative control (BM−). ALP activity of OPC1 treated with vitamin D3 and BM+ was significantly higher than BM− but not higher than the BM+ control containing ethanol vehicle. All cellular groups containing BM+ had a significantly higher ALP activity compared to the control within the MC3T3-E1 cell line. MC3T3-E1 response to vitamin D3 was indistinguishable from the response to 1,25OH2D3.
