Extrahepatic 25-Hydroxylation of Vitamin D<sub>3</sub> in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development

<table>Semi-quantification of matrix mineralization of cultured experimental groups shown in Figures <a href="../fig6/">6</a> and <a href="../fig7/">7</a> after two weeks of treatment. OPC1 treated with vitamin D<sub>3</sub> or 1,25OH<sub>2</sub>D<sub>3</sub> in BM+ had a statistically significant (<svg height="11.375" id="M39" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>) amount of calcium deposition in comparison to both BM− and BM+ controls. MC3T3-E1 cells cultured with 1,25OH<sub>2</sub>D<sub>3</sub> in BM+ had a statistically significant <svg height="15.0875" id="M40" style="vertical-align:-2.3205pt" version="1.1" viewbox="0 0 86.787498 15.0875" width="86.787498" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> amount of calcium deposition in comparison with both BM− and BM+ controls. </table>

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fig8

Figure 8

Figure 8: Extrahepatic 25-Hydroxylation of Vitamin D<sub>3</sub> in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development 

Figure 8 | Extrahepatic 25-Hydroxylation of Vitamin D3 in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development 