Extrahepatic 25-Hydroxylation of Vitamin D<sub>3</sub> in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development

<table>Endogenous production of 1,25OH<sub>2</sub>D<sub>3</sub> by OPC1 in response to incubation with vitamin D<sub>3</sub>. A 1,25OH<sub>2</sub>D<sub>3</sub>/VDR ELISA was performed to analyze the metabolism of precursor vitamin D<sub>3</sub> to the hormonally active form. The dose of vitamin D<sub>3</sub> was controlled and administered at logarithmic concentration levels. A log-base-10 model was applied to the resulting response data. </table>

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Figure 9

Figure 9: Extrahepatic 25-Hydroxylation of Vitamin D<sub>3</sub> in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development 

Figure 9 | Extrahepatic 25-Hydroxylation of Vitamin D3 in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development 