International Scholarly Research Notices

Research Article

Extrahepatic 25-Hydroxylation of Vitamin D3 in an Engineered Osteoblast Precursor Cell Line Exploring the Influence on Cellular Proliferation and Matrix Maturation during Bone Development

Table 1

ALP activity of OPC1 and of MC3T3-E1 cells maintained in six types of tissue culture medium experimental groups over three two-week periods ( ). After two weeks, both the OPC1 and MC3T3-E1 ALP activity values of 1,25OH2D3 and osteogenic factors (BM+) treated cells were statistically greater than the negative control (BM−). ALP activity of OPC1 treated with vitamin D3 and BM+ was significantly higher than BM− but not higher than the BM+ control containing ethanol vehicle. All groups containing BM+ had a statistically greater ALP activity compared to the control in the MC3T3-E1 cell line. Also, the MC3T3-E1 response to vitamin D3 treatment was indistinguishable from the response to 1,25OH2D3 treatment.
Maximum ALP activity (mean % [SEM])
Cell typeExperimental groupCulture time (days)
  259/1014/15
OPC1BM− control6.95 [4.29]12.49 [2.53]15.73 [4.64]21.39 [6.16]
BM− vitaD37.59 [4.60]21.27 [8.72]22.45 [6.60]34.87 [11.29]
BM− 1,25OH2D36.15 [2.48]11.21 [0.42]15.63 [2.48]31.90 [7.05]
BM+ control8.99 [4.87]19.20 [5.92]40.23 [4.71]69.11 [12.02]
BM+ vitaD39.63 [5.42]11.11 [2.86]21.48 [10.74]62.90 [15.56]
BM+ 1,25OH2D35.07 [1.04]18.77 [7.70]54.10 [22.78]99.81 [0.14]
MC3T3-E1BM− control1.41 [0.79]4.44 [1.37]5.31 [1.39]26.21 [5.04]
BM− vitaD32.72 [0.53]7.83 [3.13]13.46 [3.81]32.44 [10.10]
BM− 1,25OH2D32.82 [1.50]9.18 [3.80]13.79 [6.00]40.29 [14.42]
BM+ control4.13 [2.33]11.94 [7.78]22.67 [6.74]81.17 [9.74]
BM+ vitaD32.25 [0.90]15.76 [8.26]24.95 [7.42]88.24 [6.12]
BM+ 1,25OH2D32.55 [1.37]13.19 [5.03]42.76 [13.35]84.03 [8.71]