Figure 4: L1CAM and SSEA-5 immunofluorescence persists in differentiating iPSCs. IPSCs prior to differentiation (day 0) and after twenty days of retinal differentiation (day 20) were immunofluorescently labeled with both SSEA-5 (red) and L1CAM (green). For each condition, the same microscopic field is shown for L1CAM, SSEA-5, and a merged image. Note the colocalization of SSEA-5 and L1CAM in the merged images. In addition, dual-labeled SSEA-5 and L1CAM cells remain in the differentiated iPSC population after twenty days of retinal differentiation. Scale bar = 15 microns.