Research Article
Insulin Deprivation Decreases Caspase-Dependent Apoptotic Signaling in Cultured Rat Sertoli Cells
Table 1
Oligonucleotides and cycling conditions for PCR amplification of p53, bcl-2, Bax, caspase 9, caspase 3, and 18S.
| Gene | Primer sequences | AT | C | Size (bp) |
| p53 | Forward—CTG CCC ACC ACA GCG ACA GG | 59°C | 35 | 471 | Reverse—AGG AGC CAG GCC GTC ACC AT | Bcl-2 | Forward—GGG CTA CGA GTC GGA TAC | 53°C | 35 | 64 | Reverse—AGG CTG GAA GGA GAA GAT G | Bax | Forward—CGC GTG GTT GCC CTC TTC TAC TTT | 59°C | 35 | 124 | Reverse—CAA GCA GCC GCT CAC GGA GGA | Caspase 9 | Forward—TGC AGG GTA CGC CTT GTG CG | 61°C | 35 | 130 | Reverse—CCT GAT CCC GCC GAG ACC CA | Caspase 3 | Forward—AGG CCT GCC GAG GTA CAG AGC | 61°C | 35 | 255 | Reverse—CCG TGG CCA CCT TCC GCT TA | 18s | Forward—AAG ACG AAC CAG AGC GAA AG | 56°C | 25 | 149 | Reverse—GGC GGG TCA TGG GAA TAA |
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AT: annealing temperature; C: number of cycles during exponential phase of amplification.
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