Reduced antigen uptake and eosinophils influx in TSLPR^−/− mice upon OVA induced allergic asthma model. Dendritic cells were differentiated in vitro from naive bone marrow derived cells. Uptake of OVA-FITC by dendritic cells after 2 h was analyzed by FACS (100 g/mL). The data are given as the mean fluorescence intensity (MFI). OVA peptide specific T cell proliferation was assessed by coculture of DC from WT or TSLPR^−/− mice loaded with OVA peptide (10 g/mL) with CFSE labelled CD4⁺ OT2 T cells (b). Critical role of TSLPR signalling for allergic inflammatory cell recruitment in BALF in OVA immunized and challenged mice (c). OVA sensitized WT and TSLPR^−/− mice were challenged three times with OVA instillation. 24 h after the third challenge, eosinophil, lymphocyte, macrophage, and neutrophil recruitment in BAL was determined. These experiments were performed twice ( mice per group). One representative experiment is shown. Values are the mean SEM. of 8 mice per group.