Increased pulmonary IL-17A⁺ cell populations in the absence of TSLPR. Lung mononuclear cells from OVA or HDM sensitized and challenged WT and TSLPR^−/− mice were isolated and restimulated for 4 h with PMA (50 ng/mL) and ionomycin (750 ng/mL) followed by membrane staining of TCR and TCR. Representative dot plot, frequency and absolute numbers of IL-17A⁺ producing T cells gated either on TCR or TCR T cell populations (a–e) are shown for OVA model. Representative dot plot and the frequency of IL-17A⁺ producing cells gated on TCR T cell populations (f, g), IL-17A (h), IL-12p40 (i), IL-10 (j) and IFNγ (k) levels in lung supernatant from HDM treated WT and TSLPR^−/− mice are shown. Values are the mean ± SEM. of 6–8 mice per group.