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Research Article

Comparison of Lipase Production by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361 Using Fish Waste as Substrate: Optimization of Culture Conditions by Response Surface Methodology

Table 1

Composition of medium used for lipase production by MTCC 5695 and MTCC 11361.
ConstituentsAmount (g/100 mL)
Magnesium sulphate0.01
Manganese sulphate0.005
Dipotassium hydrogen phosphate0.2
Sodium acetate0.5
Ammonium citrate0.2
Tween 800.1
FWO*A# (%v/v)
FWPH**B# (mg/ml)
As replacement for carbon source.
**As replacement for nitrogen source.
#Added as per concentrations indicated in Tables 2 and 4 (protein concentration of FWPH: 33.53 mg/mL as estimated by Biuret’s method).
FWO: fish waste oil; oil recovered from fermented fish processing waste.
FWPH: fish waste protein hydrolysates; obtained after fermentation of fish processing waste.