Identifying Sources of Hepatic Lipogenic Acetyl-CoA Using Stable Isotope Tracers and NMR
Figure 2
(a), (b) 13C NMR signals of mouse hepatic fatty acids following ingestion of a [1-13C]glucose and [U-13C]fructose mixture (a) and urinary N-acetyl PABA from the same animal (b). The fatty acid penultimate and terminal 13C signals, centered at 22.7 and 14.1 ppm, respectively, consist of singlet (S) and doublet (D) components reflecting singly and uniformly enriched 13C-acetyl units of the fatty acids. The N-acetyl PABA carbon 1 and 2 resonances (C1 and C2) also consist of singlet and doublet components reflecting singly- and uniformly-enriched acetyl-CoA [7].
