A Colorimetric Method for Monitoring Tryptic Digestion Prior to Shotgun Proteomics
Figure 1
Agilent Bioanalyzer protein 80 electrophoregram of undigested and digested plasma (a) and serum (b). Although albumin is extensively digested within 8 h there are protein peaks still visible in the 24 h digested samples especially around the 20 kDa and 28 kDa range. Red line is 0 h, blue line is 8 h, and green line is 24 h.