Average protein sequence coverage for albumin, haptoglobin, and serotransferrin in serum and plasma after tryptic digestion for 2 h, 8 h, and 24 h. In serum, the average sequence coverage in 8 h and 24 h digested sample was 13.5% lower than in 2 h, and in plasma the average coverage in 8 h was 13% lower than in 2 h, and average coverage in 24 h was 22% lower than in 2 h.