International Journal of Proteomics

Research Article

A Colorimetric Method for Monitoring Tryptic Digestion Prior to Shotgun Proteomics

Table 1

Percentage of proteins digested calculated with ProDM, total number of unique peptides sequenced, and proteins identified by mass spectrometry in plasma and serum after tryptic digestion for 8 h and 24 h at 37°C.
Compared parameterPlasmaSerum
8 h24 h8 h24 h
% protein digested calculated with ProDM 46.556.146.250.2
No. of peptides sequenced by LC/MS/MS991733897693
Total no. of unique proteins identified125118127107