Research Article

The Histochemical Characterization of the Glycoconjugates in the Epidermal Mucous Cells of the Red Californian Earthworm, Eisenia foetida

Table 2

Histochemical staining properties of glycoconjugates in epidermal mucous cells of Eisenia foetida.

Body regions illustrated in Figure 1
TechniqueFirst SecondThirdFourthFifth Sixth

PAS4-54-54-53-43-43-4
AB pH 0.52-32-32-3444
AB pH 1.02-32-32-32-32-32-3
AB pH 2.54-5334-54-54-5
PAS/AB pH 2.5AB* 
PAS**
AB* 
PAS**
AB* 
PAS**
AB* 
PAS**
AB* 
PAS**
AB* 
PAS**
AF1-21-21-21-21-21-2
AF/AB pH 2.5AB+AB+AB+AB+AB+AB+
KOH/PAS4-54-54-5333

Reaction intensity: 0: negative; 1: weaker; 2: weak; 3: moderate; 4: strong; 5: stronger; *alcian blue dominant (in PAS/AB pH 2.5 staining, it refers mostly to AB (+) cells); **PAS dominant; +alcian blue dominant (in AF/AB pH 2.5 staining, it refers mostly to AB (+) cells).