In Vitro Characterization of Valproic Acid, ATRA, and Cytarabine Used for Disease-Stabilization in Human Acute Myeloid Leukemia:
Antiproliferative Effects of Drugs on Endothelial and Osteoblastic Cells and Altered Release of Angioregulatory Mediators by Endothelial Cells
Figure 3
Effects of VPA and ATRA on endothelial cell migration. (a) HUVECs were treated for 3 days with VPA-concentrations ranging from 0.3 mM to 2.4 mM and ATRA-concentrations from 0.01 μM to 1 μM before cell migration through 8 μm pore filters was determined using a transwell assay. Results are shown as mean migrated cells ± SEM (percent migrated cells compared to control cultures) for three independent experiments (*, Tukey’s multiple test). (b) Images show Hoechst-stained migrated cells on transwell filters for the highest drug concentrations of VPA and ATRA resulting in significantly altered cell migration. A representative drug-free control image for comparison with treated cultures is shown.
