Chinese Journal of Biology

Review Article

Metagenomics: An Application Based Perspective

Biocatalysts screened from metagenomic libraries by function-based approach.


Function	Habitat	Library type	Substrate	Reference

Alkaline pectate lyase	Soil	Plasmid	Pectin	[41]
Amylase	Soil	Cosmid	Starch	[43]
-amylase	Soil	Fosmid	Starch	[44]
Cellulase	Soil	Plasmid	Carboxymethyl-cellulose	[15]
Cellulase	Soil	Fosmid	Hydroxyethyl cellulose	[14]
Endocellulase	Soil	Plasmid	Carboxymethyl-cellulose	[13]
Endoglucanase	Rice straw compost	λ phage	Carboxymethyl-cellulose	[12]
Lipase	Marine sediment	Plasmid	p-Nitrophenyl (pNP) esters	[22]
Lipase	Marine sponge (Haliclonasimulans)	Fosmid	Tributyrin agar	[25]
Lipase		Fosmid	p-Nitrophenyl (pNP) esters	[26]
Lipase	Soil	Fosmid	Tributyrin agar	[27]
Lipase	Sediment	Fosmid	Triolein	[29]
Pectinase	Lagoon	Plasmid	Polygalacturonic acid	[40]
Protease	Soil	Plasmid	Azocasein	[36]
Serine proteases	Soil	Plasmid	Skim milk agar	[66]
Xylanase/endoglucanase	Sand Yak rumen	Cosmid	Xylan/CMC	[30]
Xylanase		Fosmid	Birch wood xylan	[31]
Xylanase	Holstein cattle	Fosmid	Xylan	[32]
Xylanase	rumen	Plasmid	Xylan	[23]
Xylanase	Compost soil Rumen	BAC	Oat spelt xylan	[34]