Neural crest cis-regulatory analysis in the chick embryo. Putative enhancers are identified by virtue of the conserved nature of noncoding DNA in intergenic region in proximity to neural crest genes like FoxD3, Sox10, and so forth. These putative regulatory regions are cloned upstream of a GFP-encoding construct with a basal (TK) promoter. Constructs are then electroporated into gastrula stage chick embryos and analyzed one or more days later. Those with neural crest enhancing activating drive GFP expression in migrating neural crest cells.