Research Article
Error Rate Comparison during Polymerase Chain Reaction by DNA Polymerase
Table 2
Error rate values for six PCR enzymes included in this study are presented. Each enzyme was used in two independent PCR reactions. Average doublings/PCR reaction () is the average of doubling values for each of the 94 PCR reactions in one plate, where doublings are calculated from the formula = (ng DNA after PCR/ng DNA input). Error rate (f) is calculated as f = / (target size × ), where is the number of mutations observed for all clones that were sequenced and the (target size × for each target that was cloned.
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N.D.: not determined. |