GFL reduce NO synthesis and expression of iNOS in cultivated microglial cells. Microglial cells were pretreated with GFL and afterwards activated with 5 ng/mL LPS. Concentration of nitrite ((a) OD read at 550 nm) in native microglia cell cultures (white bars), microglia stimulated with LPS alone (black bars), with GFL alone (yellow bars), or LPS in combination with GFL members (green) after 24 hrs. The NO reduction is due to downregulation of iNOS (b). Expression of mRNA was analyzed after 6 hrs using TaqMan qPCR compared with the LPS sample. 18sRNA was used as an internal control. Asterisks (, , and ) indicate a significant difference compared with cells stimulated only with LPS (ANOVA, followed by the Bonferroni test, , ).