Adaptogenic Activity of Lyophilized Hydroethanol Extract of Pandanus odoratissimus in Swiss Albino Mice
Table 1
Observation (AOT) report on acute oral toxicity test.
Sample: hydroethanol extract of Pandanus odoratissimus
Test guideline OECD 425 (modified October 2006) limit test
Animal used: Albino Swiss mice
Sex: female albino Swiss mice
Weight range: 18–25 gm
Route of drug administration: per oral
Dose: 2000 mg/kg
Fasted for 3-4 hrs (before experimentation) and 1-2 hrs (after experimentation)
No. of animal used
Date of testing
3
3
Observations
1/2 hr
1 hr
2 hr
4 hr
Awareness
4
4
4
4
Mood
0
0
0
0
Motor activity
6
6
6
6
Neurological profile
(a) Central excitation
0
0
0
0
(b) Muscle tone
4
4
4
4
(c) Motor incoordination
0
0
0
0
(d) Reflexes
4
4
4
4
(f) Autonomic profile
4
4
4
4
Screening sign
(a) Urination
0
0
0
0
(b) Salivation
0
0
0
0
General sign
Skin color
4
4
4
4
Heart rate
4
4
4
4
Respiratory rate
4
4
4
4
Death
—
—
—
—
Inference: at 2000 mg/kg sample did not produce any observable toxic effects during entire duration of study and all animals survived 14 days of observation.
