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Chromatography Research International
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Special Issues
Chromatography Research International
/
2014
/
Article
/
Tab 4
/
Research Article
Identification and Quantification of Aldose Reductase Inhibitory Flavonoids in Herbal Formulation and Extract of
Gymnema sylvestre
Using HPLC-PDA and LC-MS/MS
Table 4
Results of robustness study.
Parameters
Tested levels
Resolution between quercetin
and kaempferol
Tailing factor
Rutin
Quercetin
Kaempferol
Column temperature (°C)
35
3.27
1.44
1.43
1.45
45
3.57
1.22
1.43
1.43
Flow rate (mL/min)
0.9
3.51
1.36
1.43
1.46
1.1
3.48
1.28
1.47
1.44
Buffer concentration (mM)
5
3.48
1.35
1.43
1.47
15
3.43
1.34
1.43
1.48
pH of buffer
4.2
3.34
1.35
1.45
1.49
3.8
3.30
1.37
1.49
1.42
