International Journal of Proteomics

Research Article

Mapping and Identification of the Urine Proteome of Prostate Cancer Patients by 2D PAGE/MS

Table 2

List of the all protein spots from the PCa urinary proteome identified by MS.


Spot number	Protein name	SwissProt accession number	Mw (kDa)	pI	Mascot protein score	value	Number of the matched peptides	% of sequence coverage

1	Uromodulin OS = Homo sapiens GN = UMOD PE = 1 SV = 1	UROM_HUMAN	72.64	5.05	158		18	22
2–14, 19–21	Serotransferrin OS = Homo sapiens GN = TF PE = 1 SV = 3	TRFE_HUMAN	79.29	6.8	243		22	40
15–18	Alpha-1B-glycoprotein OS = Homo sapiens GN = A1BG PE = 1 SV = 4	A1BG_HUMAN	54.79	5.56	93		12	35
22–24	Serum albumin OS = Homo sapiens GN = ALB PE = 1 SV = 2	ALBU_HUMAN	71.31	5.92	246		26	48
25–28	Alpha-1-antichymotrypsin OS = Homo sapiens GN = SERPINA3 PE = 1 SV = 2	AACT_HUMAN	47.79	5.33	82		12	31
25–28	Kininogen-1 OS = Homo sapiens GN = KNG1 PE = 1 SV = 2	KNG1_HUMAN	72.99	6.34	82		13	19
29–33	Ig alpha-1 chain C region OS = Homo sapiens GN = IGHA1 PE = 1 SV = 2	IGHA1_HUMAN	38.49	6.08	62		5	18
34–37	Alpha-2-HS-glycoprotein OS = Homo sapiens GN = AHSG PE = 1 SV = 1	FETUA_HUMAN	40.00	5.43	95		15	43
38–43	Alpha-1-antitrypsin OS = Homo sapiens GN = SERPINA1 PE = 1 SV = 3	A1AT_HUMAN	46.89	5.37	194		23	53
44–46	Vitamin D-binding protein OS = Homo sapiens GN = GC PE = 1 SV = 1	VTDB_HUMAN	54.52	5.40	135		12	50
47	Antithrombin-III OS = Homo sapiens GN = SERPINC1 PE = 1 SV = 1	ANT3_HUMAN	53.02	6.32	85		8	21
48	Alpha-amylase 1 OS = Homo sapiens GN = AMY1A PE = 1 SV = 2	AMY1_HUMAN	58.41	6.47	82		6	15
49, 67–69	Fibrinogen beta chain OS = Homo sapiens GN = FGB PE = 1 SV = 2	FIBB_HUMAN	56.57	8.54	62		8	19
50-51	U3 small nucleolar RNA-associated protein 18 homolog OS = Homo sapiens GN = UTP18 PE = 1 SV = 3	UTP18_HUMAN	62.42	8.93	75		6	13
52–54	Leucine-rich alpha-2-glycoprotein OS = Homo sapiens GN = LRG1 PE = 1 SV = 2	A2GL_HUMAN	38.38	6.45	66		4	18
55	Fibrinogen gamma chain OS = Homo sapiens GN = FGG PE = 1 SV = 3	FIBG_HUMAN	52.10	5.37	164		17	47
56	Thymidine phosphorylase OS = Homo sapiens GN = TYMP PE = 1 SV = 2	TYPH_HUMAN	50.32	5.36	64		5	25
57–62, 118–120	Haptoglobin OS = Homo sapiens GN = HP PE = 1 SV = 1	HPT_HUMAN	45.86	6.13	126		12	27
63	Gelsolin OS = Homo sapiens GN = GSN PE = 1 SV = 1 (fragment)	GELS_HUMAN	86.04 (52.48)	5.90 (5.34)	80		7	16
64	Apolipoprotein A-IV OS = Homo sapiens GN = APOA4 PE = 1 SV = 3	APOA4_HUMAN	45.37	5.28	82		8	22
65-66	Actin, cytoplasmic 1 OS = Homo sapiens GN = ACTB PE = 1 SV = 1	ACTB_HUMAN	42.05	5.29	64		7	24
65-66	Actin, cytoplasmic 2 OS = Homo sapiens GN = ACTG1 PE = 1 SV = 1	ACTG_HUMAN	42.05	5.29	64		7	24
70-71	Fibrinogen alpha chain OS = Homo sapiens GN = FGA PE = 1 SV = 2 (fragment)	FIBA_HUMAN	95.65 (50)	5.7 (4.65)	91		10	15
72–75	Zinc-alpha-2-glycoprotein OS = Homo sapiens GN = AZGP1 PE = 1 SV = 2	ZA2G_HUMAN	34.46	5.71	104		8	29
76	Endonuclease domain-containing 1 protein OS = Homo sapiens GN = ENDOD1 PE = 1 SV = 2	ENDD1_HUMAN	55.72	5.55	104		9	20
77	E3 ubiquitin-protein ligase rififylin OS = Homo sapiens GN = RFFL PE = 1 SV = 1	RFFL_HUMAN	41.74	5.33	59		5	26
78-79	Inter-alpha-trypsin inhibitor heavy chain H4 OS = Homo sapiens GN = ITIH4 PE = 1 SV = 4 (fragment)	ITIH4_HUMAN	103.52 (45)	6.51 (5.15)	88		11	13
80	Quinone oxidoreductase-like protein 1 OS = Homo sapiens GN = CRYZL1 PE = 1 SV = 2	QORL1_HUMAN	39.07	5.49	66		4	12
81	Interleukin enhancer-binding factor 2 OS = Homo sapiens GN = ILF2 PE = 1 SV = 2	ILF2_HUMAN	43.26	5.19	62		5	19
82	Vesicular integral-membrane protein VIP36 OS = Homo sapiens GN = LMAN2 PE = 1 SV = 1	LMAN2_HUMAN	40.54	6.46	80		7	21
83	Transmembrane and immunoglobulin domain-containing protein 1 OS = Homo sapiens GN = TMIGD1 PE = 2 SV = 1	TMIG1_HUMAN	29.62	8.07	62		6	20
84–88	Protein AMBP OS = Homo sapiens GN = AMBP PE = 1 SV = 1	AMBP_HUMAN	39.87	5.95	130		15	37
89–92	Prostaglandin-H2 D-isomerase OS = Homo sapiens GN = PTGDS PE = 1 SV = 1	PTGDS_HUMAN	21.24	7.66	64		8	33
93–97	Ig kappa chain C region OS = Homo sapiens GN = IGKC PE = 1 SV = 1 (immunoglobulin kappa light chain VLJ region)	IGKC_HUMAN	28.82	6.73	77		7	29
98–102	Apolipoprotein A-I OS = Homo sapiens GN = APOA1 PE = 1 SV = 1	APOA1_HUMAN	30.75	5.56	116		16	46
103	Basement membrane-specific heparan sulphate proteoglycan core protein (perlecan) chain A, laminin-G-like domain 3 from human perlecan	PGBM_HUMAN	468.83 (20.65)	6.06 (5.47)	204		15	88
104-105	Ras-related protein Rab-36 OS = Homo sapiens GN = RAB36 PE = 2 SV = 2	RAB36_HUMAN	36.81	8.05	63		4	23
106	Tumor protein D52 OS = Homo sapiens GN = TPD52 PE = 1 SV = 2	TPD52_HUMAN	24.35	4.79	63		4	22
107	Alpha-1-acid glycoprotein 1 OS = Homo sapiens GN = ORM1 PE = 1 SV = 1	A1AG1_HUMAN	23.72	4.93	64		4	21
108–111	Retinol-binding protein 4 OS = Homo sapiens GN = RBP4 PE = 1 SV = 3	RET4_HUMAN	23.34	5.76	109		9	49
112–115	CD59 glycoprotein OS = Homo sapiens GN = CD59 PE = 1 SV = 1	CD59_HUMAN	14.79	6.02	61		5	28
116	Mannan-binding lectin serine protease 2 OS = Homo sapiens GN = MASP2 PE = 1 SV = 4 (chainA, human MBL-associated protein 19)	MASP2_HUMAN	77.19 (19.53)	5.44 (5.75)	127		8	37
117.121	Secreted and transmembrane protein 1 OS = Homo sapiens GN = SECTM1 PE = 1 SV = 2	SCTM1_HUMAN	27.30	7.00	75		6	22
122	Transthyretin OS = Homo sapiens GN = TTR PE = 1 SV = 1	TTHY_HUMAN	15.99	5.52	160		10	73
123–125	Uncharacterized protein KIAA2012 OS = Homo sapiens GN = KIAA2012 PE = 2 SV = 1 (fragment)	K2012_HUMAN	65.27 (50)	6.09 (6.00)	75		13	22