Research Article

Quantification of Gymnemagenin and -Sitosterol in Marketed Herbal Formulation by Validated Normal Phase HPTLC Method

Table 1

Linear regression data for the calibration curves ( ).

ParametersGymnemagenin -Sitosterol

Linearity range (ng band−1)100–1200200–1200
0.9990.998
Slope3.6174.528
Intercept645.660371.630
Confidence limit of slopea3.484–3.7494.183–4.872
Confidence limit of intercepta547.738–743.582121.295–621.958
34.89972.132

95% confidence limit. bStandard deviation of residuals from line.
To ascertain linearity, residual analysis was performed (Figure 2). Slope was significantly different from zero.