Chromatography Research International

Research Article

Quantification of Gymnemagenin and -Sitosterol in Marketed Herbal Formulation by Validated Normal Phase HPTLC Method

Table 4

Robustness testing ( , 200 ng band−1).
ParameterSD of peak area% RSD
Gymnemagenin -Sitosterol Gymnemagenin -Sitosterol
Mobile phase (toluene) composition ( 0.1 mL)6.405.850.450.46
Amount of mobile phase ( 5%)7.838.970.560.71
Time from band application to chromatography (+10 min)8.209.510.580.76
Time from chromatography to scanning (+15 min)8.919.110.640.73
RSD is the relative standard deviation.