Research Article

Search for Conditions to Detect Epigenetic Marks and Nuclear Proteins in Immunostaining of the Testis and Cartilage

Figure 3

Localization of methylated H3K9, acetylated H3K9, G9a, and PCNA in chondrocytes at embryonic day 16.5. Confocal images of immunohistochemical detection of H3K9me1, H3K9me2, H3K9me3, H3K9Ac, G9a, and PCNA in chondrocytes at embryonic day 16.5 are shown. A whole embryo was fixed (a) or an isolated humerus was fixed (b), embedded in paraffin, and sectioned along the longitudinal plane of the humerus. Sections were treated with antigen retrieval (AR (+)) or without (AR (−)). Scale bar = 10  m.
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