Identification of new antiresorptives by disrupting binding between microfilaments and the B2-subunit. (a) V-ATPases bind microfilaments through an actin binding site in the B-subunit. Here we have depicted a conformational change in an EG stator arm to allow access to the actin binding site. Alternatively, the EG may disassemble. (b) Experimental evidence suggested that a molecule that bound the B-subunit and prevented interaction with microfilaments might prevent trafficking of V-ATPases to the ruffled plasma membrane and thereby block bone resorption.