Proposed mechanism by which binding of microfilaments by V-ATPase can be linked to actin polymerization stimulated by the WASH complex [218]. (A) V-ATPases in a vesicle (or any membrane) may recruit the (B) WASH complex which then stimulates actin polymerization. (C) Actin filaments elongate by addition of monomers at the boundary of the membrane. (C) and (D) V-ATPases bind to an actin in the polymerizing filament and are drawn away from the original membrane as the filament elongates. (D) At some point the V-ATPase-rich membrane separates from the mother vesicle to form a new vesicle. (E) Actin filaments depolymerize and the WASH complex is released.