Research Article
Association of IL10-1082 and IFN-γ+874 Polymorphisms with Cervical Cancer among Tunisian Women
Table 1
Primer sequences used in polymerase chain reactions with specific sequence primers for the detection of cytokine gene polymorphisms.
| | Cytokine polymorphisms | | Gene sequence |
| | Interferon-gamma (intron1 +874) | Common primer | 5′-TCA ACA AAG CTG ATA CTC CA-3′ | | T allele primer | 5′-TTC TTA CAA CAC AAA ATC AAA TCT-3′ | | A allele primer | 5′-TTC TTA CAA CAC AAA ATC AAA TCA-3′ |
| | Interleukin-10 (promoter −1082) | Common primer | 5′-CAG TGC CAA CTG AGA ATT TGG-3′ | | G allele primer | 5′-CTA CTA AGG CTT CTT TGG GAG-3′ | | A allele primer | 5′-CTA CTA AGG CTT CTT TGG GAA-3′ |
| | Internal control | Primer1 | 5′-GCC TTC CCA ACC ATT CCC TTA-3′ | | Primer2 | 5′-TCA CGG ATT TCT GTT GTG TTT C-3′ |
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