Detection of Q Fever Specific Antibodies Using Recombinant Antigen in ELISA with Peroxidase Based Signal Amplification
Figure 1
SDS-PAGE profile of the purification of rCom1 protein. rCom1 expressed in BL21 (DE3) and the fractions of the nickel-column were analyzed by electrophoresis. Lane 1, molecular weight marker; Lane 2, starting sample applied onto column; Lane 3, fraction of flow through; Lane 4, fraction of wash; Lane 5 to 10, fractions of the eluate with 25, 50, 100, 200, 400, and 600 mM imidazole.
