International Journal of Bacteriology

Research Article

Detection of Q Fever Specific Antibodies Using Recombinant Antigen in ELISA with Peroxidase Based Signal Amplification

Table 2

Comparison of the standard ELISA and amplified ELISA with rCom1 and IFA with whole cell antigens (WC Ib and WC IIb) in normal human serum samples (C1 and C2) and patient samples (P1 to P6).
 ELISA (O.D.)aAmplified ELISA (O.D.)aIFA (titers)
SampleIgGIgGIgMIgGIgM
 rCom1rCom1rCom1WC IWC IIWC IWC II
C10.0560.1900.530N/AN/AN/AN/A
C20.0740.2030.536N/AN/AN/AN/A
P10.0991.1050.73210245124040
P20.0440.6190.658321281010
P30.0480.6111.4341281281010
P40.4891.8131.10710242048320640
P50.0690.8080.6995125124020
P60.0700.6580.0565125121010
 aThe values represent the averages of two experiments.
bWC I: phase I whole cell antigen; WC II: phase II whole cell antigen.