Advances in Anatomy
Volume 2014 (2014), Article ID 798425, 9 pages
http://dx.doi.org/10.1155/2014/798425
Research Article

Effect of Ultrahigh Frequency Radiation Emitted from 2G Cell Phone on Developing Lens of Chick Embryo: A Histological Study

1Department of Anatomy, Mahatma Gandhi Medical College & Research Institute (MGMC&RI), Cuddalore-Pondy Main Road, Pillaiyarkuppam, Puducherry 607402, India
2Department of Anatomy, Sri Lakshminarayana Institute of Medical Sciences (SLIMS), Bharath University, Puducherry 605502, India

Received 12 June 2014; Revised 30 August 2014; Accepted 4 September 2014; Published 17 September 2014

Academic Editor: Marius Raica

Copyright © 2014 Mary Hydrina D'Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A Mobile phone in operation emits a pulsed radiofrequency electromagnetic field which is absorbed into the user’s body particularly the head region. Contradictory scientific reports on the health effect of nonionizing radiations on biological tissues have prompted to undertake the present study to evaluate the damage in the developing lens of a chick embryo following exposure to radiation emitted from a 2G cell phone. Fertilized chick embryos were incubated in two groups in a standard egg incubator. The experiment group was exposed to radiation emitted from a 2G cell phone. On completion of scheduled duration, the embryos were collected and processed for routine histological studies. The 9th to 12th day chick embryo eyes were processed for assessment of DNA damage using the alkaline comet assay technique. The lens thickness and the equatorial diameter were measured using oculometer and statistically compared for both groups. In the present study, the exposure of chick embryos to a 2G cell phone caused structural changes in lens epithelial cells, formation of cystic cells and spaces, distortion of lens fibers, and formation of posterior aberrant nuclear layer. The DNA damage in the developing eyes of the experiment group assessed by comet assay was highly significant.