Chromatography Research International

Research Article

Development and Validation of a Rapid Chemometrics Assisted RP-HPLC with PDA Detection Method for the Simultaneous Estimation of Pyridoxine HCl and Doxylamine Succinate in Bulk and Pharmaceutical Dosage Form

Table 1

CCD consists of experiments for the study of three experimental factors with the results.


Std.	Run	Type	Factor 1	Factor 2	Factor 3	Response 1	Response 2
Std.	Run	Type	: methanol (%)	: flow rate (mL/min)	: buffer molarity (mM)	Retention time (tR₂)	Resolution (Rs₂)

1	17	Fact	40.00	0.60	40.00	12.794	25.821
2	4	Fact	50.00	0.60	40.00	6.679	16.234
3	18	Fact	40.00	1.00	40.00	8.434	20.939
4	10	Fact	50.00	1.00	40.00	3.108	11.356
5	16	Fact	40.00	0.60	50.00	14.492	24.313
6	20	Fact	50.00	0.60	50.00	6.482	14.586
7	8	Fact	40.00	1.00	50.00	7.904	21.299
8	2	Fact	50.00	1.00	50.00	4.959	10.473
9	1	Axial	36.59	0.80	45.00	15.545	28.476
10	9	Axial	53.41	0.80	45.00	4.853	10.984
11	11	Axial	45.00	0.46	45.00	9.584	22.248
12	19	Axial	45.00	1.14	45.00	4.169	13.81
13	12	Axial	45.00	0.80	36.59	6.155	17.356
14	6	Axial	45.00	0.80	53.41	5.509	13.238
15	14	Center	45.00	0.80	45.00	5.985	14.124
16	13	Center	45.00	0.80	45.00	5.768	14.167
17	5	Center	45.00	0.80	45.00	5.986	14.256
18	15	Center	45.00	0.80	45.00	5.955	17.174
19	7	Center	45.00	0.80	45.00	5.973	14.141
20	3	Center	45.00	0.80	45.00	5.979	14.12