Research Article
Mimosine As Well As Serum Starvation Can Be Used for Cell Cycle Synchronization of Sheep Granulosa Cells
Table 2
Percentages of granulosa cells in S phase of the cell cycle after a variety of culture treatments in primary culture (a) and 5th passage (b).
| (a) |
| | Normally growing
| Confluency | Serum starvation | Cell cycle inhibitors | | 24 h | 48 h | 72 h | Mimosine | Nocodazole |
| | S stage | 19.8%a | 10.2%b | 8.5%b | 3.1%b | 2.2%b | 4.5%b | 3.2%b |
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|
| (b) |
| | Normally growing
| Confluency | Serum starvation | Cell cycle inhibitors | | 24 h | 48 h | 72 h | Mimosine | Nocodazole |
| | S stage | 18%a | 9.6%b | 5.5%b | 3.2%b | 2.3%b | 3.1%b | 3.3%b |
|
|
Within a row; percentages with different superscript alphabets are significant ().
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