Molecular Biology International

Research Article

Expression of TPM1 , a Novel Sarcomeric Isoform of the TPM1 Gene, in Mouse Heart and Skeletal Muscle

Table 1

Primer pairs and probes used for amplification and detection of TPM1α and/or TPM1κ.
Name Name as in Figure 1 Sequences
Mus TPM1 (+)P15′-ATGGACGCCATCAAGAAGAA-3′
Mus TPM1 (−)P25′-TTATATGGAAGTTATATCGTT-3′
Mus TPM1 (+)P35′-CTCAAGGGCACTGAAGATGAA-3′
Mus TPM1 (+)P45′-CTCGAGGAGATCGCAGCG-3′
Mus TPM1 probeP55′-CTGGAGCTGGCGGAGAAAAAGGCC-3′
Mus TPM1 probe5′-GGCGGAGGACAGCCTCCTGGCTGC-3′
Mus TPM1 UTR (−)5′-GAGTCTTGGGAGGAGTGAAG-3′