The Role of TWIST in Angiogenesis and Cell Migration in Giant Cell Tumor of Bone

<table>The effect of TWIST siRNA on VEGF expression in the mesenchymal stromal cells of GCT. (a) The expression of TWIST in untreated (UT) and TWIST knockdown (GCTKO) GCT cells was determined using qRT-PCR in triplicate. TWIST knockdown resulted in a statistically significant decrease in VEGF and VEGFR1 expression in GCT cells (<svg height="11.375" id="M6" style="vertical-align:-0.30096pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>). (b) Western blot also shows a decrease in VEGF, VEGFR1, and TWIST protein expression in GCT stromal cells transfected with TWIST siRNA compared to untreated GCT cells.</table>

Advances in Biology

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Figure 2

Figure 2: The Role of TWIST in Angiogenesis and Cell Migration in Giant Cell Tumor of Bone 