Research Article
In Silico Proteome Cleavage Reveals Iterative Digestion Strategy for High Sequence Coverage
Table 1
Theoretical upper limits of coverage upon digestion with various cleavage agents using the iMED-FASP strategy. Iterative cleavage of the proteome starting with the rarest amino acids first results in the greatest theoretical proteome coverage of 92.9%. The reversed sequence of cleavage provides a minimal improvement to theoretical proteome coverage. Peptides were filtered after each digest keeping those with MW > 5 kDa for additional digestion. The final “flowthrough” peptides were filtered keeping only sequences with at least 7 residues.
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Reversed order of cleavage starting with the most common residues instead of the rarest residues. |