TGF-β Signaling Cooperates with AT Motif-Binding Factor-1 for Repression of the α-Fetoprotein Promoter
Figure 5
Association of Smads with ATBF1. (a) Interaction of Smad3 with ATBF1. COS7 cells were transfected with Flag-Smad3 (1 μg) with Myc-ATBF1 (2 μg) in combination with ALK5ca/V5 (0.5 μg) or without it. Cell lysates were subjected to immunoprecipitation (IP) with anti-Myc antibody followed by IB with anti-Flag antibody to show the interaction of Flag-Smad3 with Myc-ATBF1. (b) Schematic presentation of ATBF1 mutants. (c–e) Interaction of each ATBF1 mutant with Smad2 or Smad3. COS7 cells were transfected with Flag-Smad2 or Flag-Smad3 (1.5 μg) with HA-ATBF1N (2 μg) (c), HA-ATBF1 M (2 μg) (d), or HA-ATBF1C (2 μg) (e). Cell lysates were subjected to IP with anti-Flag antibody followed by IB with anti-HA antibody to show the interaction of Smad2 and Smad3 with ATBF1 mutants.
