Research Article

Highly Sensitive Loop-Mediated Isothermal Amplification for the Detection of Leptospira

Figure 1

Different methods to detect LAMP products. Reaction products were visualized on GelRed stained gel (a) or visualized directly by including hydroxy naphthol blue (b) or SYBR green (c) in the reaction. A mixture of primer sets L32-5 and L41 was used in the reaction with different copies of genomic DNA. Reactions were carried out at 63°C for 60 min. Marker, 100 bp ladder, lanes 1 to 5, reaction mixture containing 100, 50, 25, and 12, and no copies of genomic DNA.
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