Research Article
Highly Sensitive Loop-Mediated Isothermal Amplification for the Detection of Leptospira
Table 2
Detection limit in normal human plasma spiked with genomic DNA.
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Estimated cp per mL of whole blood, as plasma constitutes 55% of the total blood volume. bAssuming 100% recovery for the extraction steps. cCopy numbers are based on standard curves obtained from diluted pL32 plasmid. They are the average of three independent experiments (range of detection). dND: not detected. |