Efficient In Vitro Propagation by Ex Vitro Rooting Methods of Artemisia absinthium L., an Ethnobotanically Important Plant
Table 2
Shoot amplification on MS medium supplemented with different concentrations of cytokinins + 0.1 mg/L NAA.
Plant growth regulators (mg/L)
Number of shoots (mean ± SD)
Shoot length (cm) (mean ± SD)
Kn
BAP
NAA
0.00
0.00
0.00
00.0 ± 0.00
0.0 ± 0.00
0.25
—
0.1
18.6 ± 1.03
2.6 ± 1.38
0.50
—
0.1
23.0 ± 0.28
3.9 ± 0.96
1.00
—
0.1
32.7 ± 0.46
4.4 ± 0.52
1.50
—
0.1
28.4 ± 0.21
4.1 ± 0.86
2.00
—
0.1
25.1 ± 0.20
3.7 ± 0.50
—
0.25
0.1
25.4 ± 0.94
2.8 ± 0.73
—
0.50
0.1
33.6 ± 0.22
4.3 ± 0.67
—
1.00
0.1
38.0 ± 0.54
5.6 ± 0.26
—
1.50
0.1
35.9 ± 0.82
5.0 ± 0.39
—
2.00
0.1
30.7 ± 0.29
4.5 ± 0.42
0.25
0.25
0.1
36.1 ± 0.76
4.1 ± 0.64
0.50
0.50
0.1
41.0 ± 0.52
5.6 ± 0.42
1.00
1.00
0.1
43.7 ± 0.39
7.4 ± 0.26
1.50
1.50
0.1
40.5 ± 0.22
6.0 ± 0.44
2.00
2.00
0.1
38.8 ± 0.17
5.5 ± 0.27
Note: each value represents the mean ± SD of ten replicates. Mean values ± SD followed by the same superscripts within columns are not significantly different at level by Duncan’s Multiple Range Test (DMRT).
