Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin

<div>Growth kinetics of Re-5, Re-6, and Re-9 on MDCK-TMPRSS2 and MDCK-MSPL cells. MDCK-TMPRSS2, MDCK-MSPL, MDCK + TPCK, and MDCK cells were infected with Re-5, Re-6, and Re-9 as described and samples were taken at 12, 24, 48, 72, and 96 h after infection. Sample TCID<sub>50</sub> were determined and calculated using the method of Reed and Muench.</div>

Biotechnology Research International

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Figure 5

Figure 5: Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin 

Figure 5 | Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin 