Research Article
Identification of Putative Molecular Markers Associated with Root Traits in Coffea canephora Pierre ex Froehner
Table 5
Polymorphic microsatellite primers and their sequences used to study the segregation of markers in the mapping population.
| | Serial number | Primer | Sequence | Tm (°C) | Size range |
| | 1 | CM11 | F: GCTGCCAGAAAAATGTTGCAGTG | 58 | 270–338 | | R: CTGCCTCGTAAAAGCTTGCGTTG |
| | 2 | CM13 | F: GCTATGCAGCTTGTTCGCAATCC | 59 | 350–411 | | R: CCAGCTATATCAGGAGCAGAACC |
| | 3 | CM180 | F: CATGTGTAATACATTCAACAGTGA | 60 | 300–350 | | R: GCAATAGTGGTTGTCATCCTT |
| | 4 | CM32 | F: AACTCTCCATTCCCGCATTC | 60 | 180–200 | | R: CTGGGTTTTCTGTGTTCTCG |
| | 5 | CM46 | F: CAGCTAGTGTGAAGGGAAAC | 55 | 300–350 | | R: GTTATCATGGTCTTACACG |
| | 6 | CM20 | F: CTTGTTTGAGTCTGTCGCTG | 60 | 250–300 | | R: TTTCCCTCCCAATGTCTGTA |
| | 7 | CM171 | F: TTCCCCCATCTTTTTCTTTC | 60 | 250–300 | | R: TTGTATACGGCTCGTCAGGT |
| | 8 | CM166 | F: AAGAGGTGCCTATCACCGTC | 60 | 230–260 | | R: CGAGGTATCAAAAAGCACCT |
| | 9 | CM211 | F: TCATGCCAAATATGAGTGGA | 60 | 300–350 | | R: GAGATGGCAAAGGCTGTTC |
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