Research Article

Quality Assessment of 25(OH)D, Insulin, Total Cholesterol, Triglycerides, and Potassium in 40-Year-Old Frozen Serum

Table 2

Descriptive statistics, percentage of change, and correlation for serum concentrations measured in the same 26 subjects (total cholesterol, triglycerides, and potassium) and 29 subjects (insulin), respectively, in 1968-1969 and 2012.

Baseline samples analyzed in
1968-1969 (same year)
Samples reanalyzed in 2012 Median percentage change ()b Spearman rho ()
RangeMedianMean (SD)RangeMedian Mean (SD)

Total cholesterol (mmol/L)265.1–7.96.5 6.4 (0.74)3.9–6.95.3 5.4 (0.79)−16.0***0.86***
Triglycerides (mmol/L)260.7–2.41.0 1.1 (0.43)0.8–2.61.2 1.3 (0.49)15.2***0.90***
Potassium (mmol/L)263.6–4.84.1 4.1 (0.32)3.6–5.04.2 4.2 (0.28)3.2***0.89***
Insulin (mU/L)a293.0–47.019 19.7 (10.30)1.2–31.07.8 10.5 (8.70)−54.0***0.74***

*** < 0.001.
aTo convert from mU/L to pmol/L multiply by 6.945.
bPercentage of change was calculated as (level in 2012 − level at baseline)/level at baseline * 100%. values refer to the Wilcoxon signed rank test.