Gel representing typical restriction fragment length polymorphism patterns obtained by subjecting PCR products to restriction digestion by enzyme, PagI (New England Biolabs Inc., USA). Lane 1 consists of the 100 bp DNA marker (Bangalore Genei, India). Lanes 2 and 3 represent RFLP pattern for homozygous mutant alleles (TT). Lanes 4 and 5 represent RFLP pattern for heterozygous (CT) alleles. Lanes 6 and 7 represent RFLP pattern for homozygous wild type (CC) alleles.