Research Article

Cigarette Smoking Is Associated with a Lower Concentration of CD105+ Bone Marrow Progenitor Cells

Figure 1

Cytometric analysis of cells that were positively isolated from a sample of bone marrow aspirate obtained from a 34-year-old male nonsmoker. Cells were isolated using the magnetic activated cell sorter (MACS, Miltenyi Biotec) and anti-CD105 monoclonal antibodies conjugated to a metallic residue. Cells were then stained with IgG control antibody or fluorescent monoclonal anti-CD105 antibody. Left panel shows IgG control (gated cells, 2.84%); right panel shows CD105+ cells (gated cells, 92.32%). Representative analysis for one out of 24 bone marrow samples.
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